Pharmacokinetic Study of Scutebarbatine A and Scutebarbatine B in Rats After Orally Administration of Scutellaria Barbata Ex-tract Using a LC-MS∕MS Method
10.14148/j.issn.1672-0482.2016.0479
- VernacularTitle:半枝莲提取物中半枝莲碱A和半枝莲碱B大鼠体内药代动力学研究
- Author:
Yue-Long GUO
1
;
Jin-Rui GUO
;
Lei ZHANG
Author Information
1. 江苏省省级机关医院药剂科
- Keywords:
Scutellaria barbata;
LC-MS∕MS;
pharmacokinetics
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(5):479-482
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE The aim of this study was to develop and validate a LC-MS∕MS method to simultaneously determi-nation of Scutebarbatine A and Scutebarbatine B in rat plasma and applicated this method to pharmacokinetic study of Scutebar-batine A and Scutebarbatine B after oral administration of Scutellaria barbata extract.METHODS Plasma samples were pre-pared with ethyl acetate using liquid-liquid extraction.Then samples were separated by using BDS Hypersil C1 8(50 mm×2.1 mm,2.4 μm)with gradient elution program and oven temperature was set at 40 ℃.And the mobile phase was consisted with acetonitrile∕water(0.05% formic acid) at a flow rate of 0.25 mL∕min.Determination was carried out on a tandem mass spec-trometer in positive ion mode using a multiple reaction monitoring(MRM)via a electrospray ionization(ESI)interface.After oral administration of 500 mg∕kg Scutellaria barbata extract,plasma samples were collected and analyzed by using a DAS soft-ware to analyze to the pharmacokinetic parameters.RESULTS Scutebarbatine A and Scutebarbatine B were liner at the range of 0.5 12~258.0 ng∕mL,0.482~241.0 ng∕mL,respectively with a r 2 larger than 0.994.Accuracy,precious,extraction effi-ciency,matrix effects and stability study were all meet the requirement of the bioanalytical method.The AUC(0-t) for Scutebar-batine A and Scutebarbatine B were(88.69±12.4)μg∕L.h,(57.09±9.84)μg∕L.h,respectively.And the Cmax for Scutebar-batine A and Scutebarbatine B were(10.22±1.31)ng∕mL and (6.27 ±0.80)ng∕mL.CONCLUSION The method was sim-ple,accurate,and sensitive,which could be used for the pharmacokinetic study of Scutebarbatine A and Scutebarbatine B after oral administration of Scutellaria barbata extract.
