Study on the Excretion of Liguzinediol in Rats by UPLC-PDA Method
10.14148/j.issn.1672-0482.2016.0475
- VernacularTitle:UPLC-PDA 法研究 Liguzinediol 在大鼠体内的排泄
- Author:
Bang DONG
1
;
Xiao-Qin YOU
;
Hong-Mei WEN
;
Zhen-Li DAI
;
Chen-Xiao SHAN
;
Xin-Zhi WANG
;
Wei LI
Author Information
1. 南京中医药大学药学院
- Keywords:
Liguzinediol;
metabolites;
UPLC-PDA;
rat;
excretion
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(5):475-478
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To study the excretion of Liguzinediol and its metabolites in rats by UPLC-PDA method.METH-ODS Six SD rats were administered intravenously at a dose of 10 mg∕kg.Its urine,bile and feces were collected according to certain time points.The samples were treated by methanol and the supernatant was dried by N2 ,then dissolved by mobile phase.Liguzinediol and its metabolites in urine,bile and feces were determinated by UPLC-PDA.Conversion factor was con-firmed through the determination of UV absorption coefficient and molecular weight of prototype drug together with its metab-olites.Then the cumulative excretion of Liguzinediol in rats(Dose%)was calculated.RESULTS There were slight differences in the excretion process between female and male rats.The Dose% of Liguzinediol and its main metabolites in female rats u-rine,bile and feces respectively were 47.94%,1 6.67% and 0.648%,and the total Dose% was 65.26%.The Dose% of Ligu-zinediol and its main metabolites in male rats urine,bile and feces respectively were 35.00%,20.37% and 1.1 56%,and the total Dose% was 56.53%.CONCLUSION The UPLC-PDA method with the addition of conversion factor can be used to ex-plore the material balance of Liguzinediol in rats,and provide experimental evidence for clinical research.
