Effect of Baicalin on Expression of NF-κB in Ulcerative Colitis Rats
10.14148/j.issn.1672-0482.2016.0447
- VernacularTitle:黄芩苷对溃疡性结肠炎模型大鼠 NF-κB 表达的影响
- Author:
Lei ZHU
1
;
Hong SHEN
;
Pei-Qing GU
;
Yin JIANG
;
Un Ya-j LIU
;
Lu ZHANG
;
Jia-Fei CHENG
Author Information
1. 南京中医药大学附属医院
- Keywords:
Baicalin;
ulcerative colitis;
NF-κB;
inflammation
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(5):447-450
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To explore the effect of Baicalin in vivo of the expression of NF-κB and cytokines in ulcerative coli-tis rats.METHODS The colitis model of mice were established with TNBS methord.Mice were randomly divided into control group,model group,Baicalin group,and SASP group.After continuous administration for 10 days,observed the colon gross morphological changes,the colon tissue level of TNF-α,IL-8 and IL-1 were detected using ELISA,and the protein expression of NF-κB in the colon tissues of mice were detected using Western Blot.RESULTS Compared with the model group,Baicalin(100 mg∕kg) group could significantly improve the colon gross morphological form,the Baicalin(25,50,100 mg∕kg)groups could in-hibit the secretion of inflammatory factors dose-dependently,the high dose group could reduce IL-1,IL-8 and TNF-αexpression (P <0.05),no significant difference occured between the SASP group.Compared with the model group,.Baicalin high dose group could reduce NF-kB levels,promote IkBαlevel(P <0.05) .CONCLUSION Baicalin can inhibit NF-κB activation,and thus play its anti-inflammatory effect in ulcerative colitis mice.
