Determination of two perfluorinated compounds in urine by liquid chromatography-tandem mass spectrometry
10.3760/cma.j.cn121094-20230217-00047
- VernacularTitle:尿液中2种全氟化合物测定的液相色谱串联质谱法
- Author:
Xiaohong CHEN
1
;
Qiaoli QIU
Author Information
1. 宁波市疾病预防控制中心理化检验所，浙江省微量有毒化学物健康风险评估技术研究重点实验室，宁波 315010
- Keywords:
Fluorine compounds;
Chromatography, liquid;
Tandem mass spectrometry;
Solid phase extraction;
Perfluorooctane sulfonic acid (PFOS);
Perfluorooctanoic acid
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2024;42(3):202-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of two perfluorinated compounds in urine by liquid chromatography-tandem mass spectrometry.Methods:In November 2022, urine samples were extracted by acidic methanol, purified by WAX solid phase extraction column, and eluted with methanol water, then Waters ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) was used with 1.0 mmol/L ammonium acetate solution and methanol as mobile phase. The gradient elution was carried out, the detection was carried out by electrospray negative ion multiple response monitoring (MRM) mode, and the quantitative method was internal standard method.Results:Perfluorooctanoic acid and perfluorooctane sulfonic acid had a good linear relationship in the concentration range of 0.5-50.0 μg/L, and the correlation coefficient was >0.999. The limit of detection was 0.017 μg/L, and the limit of quantitation was 0.005 μg/L. The average recoveries were 96.3% and 101.8%, respectively. Days of precision were 3.5%-6.2% and 3.1%-7.4%, respectively, daytime precision were 4.3%-6.8% and 4.7%-8.1%, respectively.Conclusion:The established method of liquid chromatography-tandem mass spectrometry is high sensitivity and accuracy, and is suitable for the determination of perfluorooctanoic acid and perfluorooctane sulfonic acid in human urine.
