Establishment and Staging Studies of Human Ovarian Cancer HO-8910 Orthotopic Xenograft Animal Models
10.14148/j.issn.1672-0482.2016.0356
- VernacularTitle:人卵巢癌 HO-8910原位移植瘤动物模型建立及分期研究
- Author:
Xiu-Xia GUO
1
;
De-Cai TANG
;
Wen-Hua ZANG
;
Min LIU
;
Jian-Guo DAI
;
Shuo ZHANG
;
Gang YIN
Author Information
1. 南京中医药大学基础医学院
- Keywords:
ovarian cancer;
HO-8910 cell line;
orthotopic xenograft;
stage
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(4):356-358,382
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish an ovarian cancer xenograft model by fluorescence and study of the cancer staging. METHODS The human ovarian cancer HO-8910 cells were cultured for 10 generations in the cell culture dish,and the cells that could stably express fluorescence were selected and injected into nude mice.When the tumor grew to a mass volume of 1 mm3 ,it was implanted into the ovarian mucous membrane of nude mice through micro-surgery.The tumor growth and metas-tasis were observed by in vivo imaging technique,and the tumor tissue and surrounding tissues were observed through patholo-gy detection methods.RESULTS In vivo imaging technology successfully traced the development and metastasis of tumor. Pathological examination showed tumor cell metastasis trend.Both of them combining with TCM clinical ovarian cancer staging determined the ovarian cancer staging.CONCLUSION The experiment successfully establishes ovarian cancer orthotopic transplantation model and represents the evolution of clinical oncology.The stages will provide some experimental basis for the study of tumor and the evaluation criterion of antitumor drug effect.
