Mechanism of thermoTRP Participating in Cold Hyperalgesia of Rat with Experimental Knee Osteoarthritis
10.14148/j.issn.1672-0482.2016.0347
- VernacularTitle:基于 thermoTRP 介导冷痛敏机制对膝骨关节炎虚寒冷痛的研究
- Author:
Run-Lin XING
1
;
Pei-Min WANG
;
Jun MAO
;
Nong-Shan ZHANG
;
Ling-Rui ZHAO
;
Xiao-Chen LI
Author Information
1. 南京中医药大学附属医院
- Keywords:
knee osteoarthritis;
deficiency cold;
cold hyperalgesia;
TRP ion channel;
occurrence mechanism
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(4):347-351
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe both TRPA1 and TRPM8 expressions in synovial tissues of rats with cold hyperalgesia induced by experimental knee osteoarthritis(KOA).METHODS 45 Sprague Dawley male rats,weight ranging from 440 g to 470 g were randomly individed into the model group,model-antagonist and normal group.Paw withdrawal latency (PWL) of five rats from each group was determined one week before modeling,2,4,6 and 8 weeks after modeling,respectively.8 weeks after modeling,the rats were killed and synovial and cartilage tissues from diseased knee were collected,to observe pathological morphology at cartilage tissues and to determine the protein and gene expressions of TRPA1 and TRPM8 at syno-vial tissues.RESULTS KOA rats showed obvious cold hyperalgesia from two weeks after modeling to the latest follow-up,8 weeks after modeling.The abnormally low level of PWL could be increased by TRPA1 and TRPM8 ion channel blocker.CON-CLUSION Up-regulating expressions of TRPA1 and TRPM8 participate in the occurrence mechanism of KOA cold hyperalge-sia.
