Study on Function of JAK2/STAT3 Pathway in Promoting Angiogenesis with Angelica Microcapsules for Replenishing Blood
10.14148/j.issn.1672-0482.2016.0342
- VernacularTitle:当归补血微囊促血管新生中 JAK2/STAT3通路的作用研究
- Author:
Un Hai-j CHEN
1
;
Li-Qun PAN
Author Information
1. 南京中医药大学第一临床医学院
- Keywords:
Angelica microcapsules for replenishing blood;
angiogenesis;
JAK2/STAT3 pathway;
VEGF;
miRNA-21
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(4):342-346
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the function of JAK2/STAT3 pathway in promoting angiogenesis with Angelica mi-crocapsules for replenishing blood (Ang-Mc).METHODS Establish the human umbilical vein endothelial cell (HUVEC) model,and then cell counting kit(CCK-8) and flow cytometry were used to test cell viability and cell apoptosis.Western blot was used to detect expression of p-STAT3 and VEGF.RT-PCR was used to detect expression of vascular endothelial growth factor(VEGF) and miRNA-21.RESULTS When Ang-Mc with a concentration of 20 μg/mL acted on HUVEC for 24 h,it could effectively promote the HUVEC proliferation by up-regulating the expression of p-STAT3,VEGF and miRNA-21. AG490 can effectively inhibit Ang-Mc promoting HUVEC proliferation effect and reduce the expression of p-STAT3,VEGF and miRNA-21.CONCLUSION The effect of Ang-Mc in promoting angiogenesis is related to JAK2/STAT3 signaling path-ways.
