Study on Serum Proteomics of Rheumatism with Damp-heat Syndrome Based on iTRAQ Labeling Method Combined with Bioinfor-matics Technology
10.14148/j.issn.1672-0482.2016.0316
- VernacularTitle:基于 iTRAQ 标记结合生物信息学技术的风湿性疾病湿热证血清蛋白组学研究
- Author:
Zhi-Ling SUN
1
;
Miao-Miao WANG
;
Xiao XU
;
Ling WANG
;
Fu-Qiang WANG
;
Wei JI
Author Information
1. 南京中医药大学护理学院
- Keywords:
ankylosing spondylitis;
gout;
damp-heat syndrome;
serum;
proteomics
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(4):316-321
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the serum proteomics of rheumatism with damp-heat syndrome by using iTRAQ la-beling method together with bioinformatics technology.METHODS 20 serum samples respectively from ankylosing spondyli-tis with damp-heat syndrome group,arthrolithiasis with damp-heat syndrome group and normal control group were collected for proteomics analysis through iTRAQ labeling and liquid chromatography.Such network database platforms as DAVID,Uni-ProtKB/Swiss Prot and IPA were resorted to perform bioinformatics analysis of differential protein in rheumatic diseases with damp-heat syndromes and verification would be done to the significant differential proteins.RESULTS Fifteen differential pro-teins of dampness-heat syndrome were obtained,with twelve being the up-regulated expression proteins and three being the down-regulated expression proteins.The five typical biological signaling pathways of most significant meaning related to differ-ential protein expression were obtained through analysis.These differential protein functions and bioinformatic analysis were proved to be related to the acute phase responses and activities of diseases.Verification test results were consistent with those of iTRAQ.CONCLUSION This provides evidences for further study on molecular mechanism of rheumatic diseases with damp-heat syndromes as well as the candidate diagnostic markers.
