Study of the Effect and Mechanism of Dermatitis Lotion in the Treatment of Allergic Contact Dermatitis in Mice
10.14148/j.issn.1672-0482.2016.0279
- VernacularTitle:皮炎洗剂对小鼠慢性变应性接触性皮炎的影响及其作用机制
- Author:
Qing LIU
1
;
Qiong WANG
;
Hui CHEN
;
Yu-Hong JIANG
;
Li-Ling WANG
;
Si-Nong WANG
Author Information
1. 甘肃中医药大学中医临床学院
- Keywords:
dermatitis lotion;
mice;
allergic contact dermatitis;
IFN-γ;
IL-4
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(3):279-282
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To study the efficacy of dermatitis lotion in the treatment of allergic contact dermatitis in mice. METHODS The 2,4-dinitro chlorobenzene(DNCB)was applied on the dorsum of mice to induce the allergic contact dermatitis models.60 mice were randomly divided into control,model(ACD),momestasone furoate,dermatitis lotion(0.4,0.2,0.1 g∕mL) groups,with 10 mice in each group.Mice were sensitized by DNCB and then treated topically with different drugs on le-sions.The inflammation degree of skin lesion were observed,histopathological analysis was measured by HE strain and the levels of serum IFN-γand IL-4 were detected by ELISA.RESULTS The dermatitis lotion of 0.4 g∕mL restrained the increase of thymus index,alleviated the inflammation degree of lesion on dorsum of mice and reduced the pathological score significant-ly,compared with the model group.In addition,the dermatitis lotion of 0.4 g∕mL adjusted the expression of serum IFN-γand IL-4 to return to normal levels.CONCLUSION These results suggest that dermatitis lotion can be used for the treatment of allergic contact dermatitis (ACD) mainly through the regulation Th1∕Th2 cytokines.
