Effects of Isoliquiritigenin on Induction of Apoptosis and Expression of ERS Related Genes in NCI-H1 57 Cells
10.14148/j.issn.1672-0482.2016.0264
- VernacularTitle:异甘草素诱导 NCI-H 157细胞凋亡及对 ERS 相关基因表达的影响
- Author:
Li LIU
1
;
Hong SHEN
;
Un Peng-j JIANG
;
Jie CHEN
;
Qing LIU
;
Xu ZHANG
Author Information
1. 南京中医药大学附属医院
- Keywords:
isoliquiritigenin;
NCI-H1 57;
proliferation;
apoptosis;
endoplasmic reticulum stress
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(3):264-268
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To study the effect of isoliquiritigenin on cell proliferation,apoptosis induction,mRNA expression of endoplasmic reticulum stress related gene ATF4,DDIT3 and TNFRSF10B in NCI-H1 57 human lung cancer cell line. METHODS NCI-H1 57 cells were cultured and treated with various concentrations isoliquiritigenin.The cell proliferation was analyzed by MTT and the occurrence of cell apoptosis was examined by flow cytometry.ATF4,DDIT3 and TNFRSF10B gene mRNA expression were detected by real-time fluorescence quantitative polymerase chain reaction.RESULTS Isoliquiritigenin significantly inhibited NCI-H1 57 cell proliferation in a dose and time dependent manner.The results of flow cytometry revealed that isoliquiritigenin could induce apoptosis in NCI-H1 57 cells.Real-time quantitative RT-PCR showed that isoliquiritigenin up-regulated mRNA expression of ATF4,DDIT3 and TNFRSF10B(P <0.05 ~0.01).CONCLUSION Isoliquiritigenin can in-hibit NCI-H1 57 cell proliferation,induce cell apoptosis by activating the endoplasmic reticulum stress response.
