Effects of Qingluo Tongbi Compound on Pain Behavior and Expression of COX-2 in Dorsal Root Ganglion and Blood PGE2
10.14148/j.issn.1672-0482.2016.0250
- VernacularTitle:清络通痹方对小鼠疼痛行为及 DRG 中 COX-2表达和血 PGE2的影响
- Author:
Ya-Mei ZHU
1
;
Ling-Ling ZHOU
;
Xiao-Wu PENG
;
Zong-Xiang TANG
;
Xue-Ping ZHOU
Author Information
1. 南京中医药大学第一临床医学院
- Keywords:
Qingluo Tongbi Compounds;
pain;
CIA;
COX-2;
PGE2
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(3):250-254
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To study the effects of Qingluo Tongbi Compound(QLT)on pain behavior and COX-2 mRNA ex-pression in dorsal root ganglion(DRG)and blood PGE2 concentration,and to explore the mechanisms of pain in rheumatoid ar-thritis.METHODS The mice were randomly divided into control group,celecoxib 30 mg∕kg,QLT 4.35 g∕kg,QLT 8.70 g∕kg and QLT 1 7.4 g∕kg groups.The mice pain threshold change were measured by hot plate method,and body torsion times were tested by acetic acid twisting method.The collagen-induced arthritis(CIA) was induced by collagenⅡ in DBA∕1 mice. The mice were randomly divided into control group,CIA group,celecoxib 30 mg∕kg group,QLT 8.70 g∕kg.The paws swell-ing,mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were recorded.After treated with corre-sponding drugs by intragastric administration for four weeks,COX-2 mRNA in DRG and PGE2 in mice were quantified.RE-SULTS QLT reduced pain threshold and body torsion times in pain model mice(P <0.01);compared with the CIA group,the QLT group decreased the paws swelling(P <0.05),up-regulated MWT(P <0.01) and PWL(P <0.05),down-regulated the expression level of COX-2 mRNA in DRG and PGE2 concentrations in blood.CONCLUSION QLT shows certain analgesic ac-tion,which might be related to the inhibitory effect of COX-2 mRNA expression in DRG and PGE2 in blood.
