Comparison of Three Kinds of Flavonoids and Total Flavonoids Contents in Different Organs ofScutellaria Baicalensis Geogi and Its Callus
10.14148/j.issn.1672-0482.2016.0191
- VernacularTitle:黄芩不同器官及愈伤组织中3种黄酮类成分和总黄酮的含量比较
- Author:
Ping ZHOU
1
;
Chen-Qiong XIE
;
Jian-Wei CHEN
;
Xiang LI
Author Information
1. 南京中医药大学药学院
- Keywords:
Scutellaria baicalensis Geogi;
different organs;
callus;
scutellarin;
baicalin;
wogonoside;
total flavonoids
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(2):191-194
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To compare the contents of three kinds of flavonoids(scutellarin,baicalin and wogonoside)and to-tal flavonoids in different organs of Scutellaria baicalensis Georgi(root,stem,leave anf flower),medicinal herbs of Scutel-laria(tender Scutellaria ,old Scutellaria and green Scutellaria)and its callus.METHODS SYMMETRY C18(4.6 mm×250 mm,5 μm) column was used with the solvent system of acetonitrile(A)-0.22% acetic acid water(B) and the UV detection wavelength of 335 nm,at the temperature of 35 ℃,to detect the contents of three kinds of flavonoids.The contents of total flavonoids were determined with the control sample of rutin,the indicators of aluminum nitrate and the UV detection wave-length of 504 nm.RESULTS The linear ranges of scutellarin,baicalin and wogonoside were 6.0~192.0,30.0~960.0,12.0~384.0μg/mL(R 2 ≤0.998 9),respectively.The average recoveries were 97.8%~103.1%,RSD ≤4.0%.The linear ranges of total flavonoids was 40.0~240.0 mg/mL(R 2 ≥0.999 8),and the average recoveries was 98.8%(RSD = 1.0%) .CON-CLUSION The content of scutellarin in aboveground Scutellaria baicalensis Georgi (flower,stems and leaves) was higher than underground part(root),and the content in flowers is highest,reaching the point of 0.53%.Baicalin and wogonoside content in underground part of are higher than aboveground,and the content in callus baicalin reaches 0.60%.In callus,the content of total flavonoid is highest(30.42%).Those results may provide a basis for scutellarin extraction from Scutellaria flower and the production of total flavonoids from Scutellaria callus.
