Effect of Peiminine on MEK1/2,ERK1/2 and the Phosphorylatioin in Lung Tissue of Pulmonary Fibrosis in Rats
10.14148/j.issn.1672-0482.2016.0170
- VernacularTitle:贝母素乙对肺纤维化大鼠肺组织 MEK1／2、ERK1／2及其磷酸化的影响
- Author:
Hai GUO
1
;
Fu-Zhi JI
;
Xiao-Feng ZHAO
;
Jie-Ning GONG
;
Jing-Lian QU
Author Information
1. 南京医科大学附属淮安第一医院
- Keywords:
peiminine;
MEK1/2;
ERK1/2;
phosphorylatioin;
pulmonary fibrosis
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(2):170-175
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To investigate the effect of peiminine on MEK1/2,ERK1/2 and the phosphorylatioin of bleomycin-induced pulmonary fibrosis in rats.METHODS Bleomycin of 5 mg/kg was instilled into the rats with a microliter injector to induce the acute lung injury.Sham-operated group and control group were given distilled water of 1 mL/100 g.Dexamethasone group was given equal volume of dexamethasone distilled water solution.Peiminine A and B group were given equal volume of peiminine distilled water solution of 5 mg/kg and 2.5 mg/kg,respectively.After consecutive daily gavage for 28 d,the rats were anesthetized and killed by carotid exsanguination.Lungs were fixed and embedded,and 4 μm sections were prepared. MEK1/2 and ERK1/2 in the lung tissues were detected by immunohistochemistry.The p-MEK1/2 and p-ERK1/2 were detec-ted by Western blot.RESULTS Peiminine significantly reduced the levels of ERK1/2 and MEK1/2 in lung tissues of rats with bleomycin-induced pulmonary fibrosis(P <0.01),and the effects was similar to dexamethasone (P > 0.05).No significant difference was observed between peimine A group and B group(P >0.05).Compared to the model group and dexamethasone group,peiminine significantly reduced the level of p-MEK1/2 and p-ERK1/2 in lung tissue of bleomycin-induced pulmonary fi-brosis rats(P <0.01).Compared to peimine A group,the B group displayed more significant efficacy(P <0.01).CONCLU-SION Peiminine attenuates the level of MEK1,ERK1 / 2 and the phosphorylation in lung tissues of bleomycin-induced pul-monary fibrosis rats,which may be one of the mechanism of anti-fibrosis.
