Effect of Shengjiang San on NF-κB Activity in LPS-activated Rat Alveolar Marcrophage Cell Line
10.14148/j.issn.1672-0482.2016.0166
- VernacularTitle:升降散对LPS诱导大鼠肺泡巨噬细胞NF-κB信号的影响
- Author:
Ming-Ming QI
1
;
Jian MA
;
Feng-Ming ZHAO
Author Information
1. 南京中医药大学基础医学院
- Keywords:
Shengjiang San;
LPS;
TRL4;
NF-κB signal
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(2):166-169
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To investigate the effect of Shengjiang San on expression of TLR4 and NF-κB in LPS-induced rat pulmonary alveolar macrophage cell.METHODS NF-κB signal pathway in NR8383 cells was stimulated with LPS.The ex-pression level of TLR4 was measured by QRT-PCR and Western Blotting separately.NF-κB activity was detected by Dual Lu-ciferase Reporter Gene Assay kit.The protein expression level of p-p65 was detected by Western Blotting.The mRNA level of TNF-α,A20,IL-6,IL-1β was measured by QRT-PCR,and the production of TNF-α,IL-6,IL-1β in the supernatant of NR8383 was determined with ELISA.Shengjiang San treated NR8383 cells,which were induced NF-κB signal pathway by LPS.NF-κB activity was detected by Dual Luciferase Reporter Gene Assay kit,qRT-PCR,Western Blotting and ELISA.RE-SULTS LPS induced TLR overpression in both mRNA and protein levels.Moreover,NF-κB signaling pathway and down-stream target cytokines were increased with dose-dependent.10 μg/mL Shengjiang San treatment abrogated the induction of LPS to TLR4 and NF-κB signaling pathway.CONCLUSION Shengjiang San significantly reduced LPS-activated NF-κB signa-ling pathway in NR8383.
