Research on the Metabolism of Ginsenoside Rb1 by Intestinal Flora under Anaerobic and Aerobic Conditions in vitro
10.14148/j.issn.1672-0482.2015.0567
- VernacularTitle:人参皂苷 Rb 1在体外肠道菌群模型中的代谢研究
- Author:
Jing QIAN
1
;
An KANG
;
Liu-Qing DI
;
Ya-Wei DI
;
Jie LI
Author Information
1. 南京中医药大学药学院
- Keywords:
ginsenoside Rb1;
anaerobic;
intestinal flora;
metabolism;
UPLC-MS/MS
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(6):567-570
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Research on the gradual deglycosylated metabolism of ginsenoside Rb1 by intestinal flora under aer-obic and anaerobic conditions in vitro .METHODS The changes of Rb1 and its metabolites were detected by means of UPLC-MS/MS incubated by intestinal bacteria under aerobic and anaerobic conditions in vitro after 0,2,4,8,12,24,48 h and 72 h in vitro .RESULTS It was found that Rb1 was converted into three metabolites ginsenoside Rd,F2 and C-K by rat intestinal bac-teria in vitro through gradual removal of glycosylation while Rd→F2 was the rate-limiting step.The metabolic pathway of Rb1 by intestinal bacteria was Rb1 →Rd→F2 →C-K.CONCLUSION The deglycosylated metabolites of Rb1 under aerobic and anae-robic conditions have no differences,but the metabolic rate under aerobic conditions is faster.
