Study of Jianpi-Bushen Fang on Treatment of Ulcerative Colitis by Promoting BMSCs Proliferation
10.14148/j.issn.1672-0482.2015.0560
- VernacularTitle:健脾补肾方促进 BMSCs 增殖治疗溃疡性结肠炎的实验研究
- Author:
Lei ZHU
1
;
Hong SHEN
;
Pei-Qing GU
;
Li LIU
;
Chang-Le ZHU
;
Hai-Peng SI
Author Information
1. 南京中医药大学附属医院
- Keywords:
ulcerative colitis;
Jianpi-Bushen Fang;
mesenchymal stem cells;
proliferation;
inflammation
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(6):560-563
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the mechanism of ulcerative colitis(UC)treatment by Jianpi-Bushen Fang through pro-moting the differentiation of BMSCs in model rats.METHODS The rats were divided into 5 groups:normal group,model group,BMSCs group,BMSCs intervene group and combine group.BMSCs intervene group and combine group were injected with decoction intervened BMSCs through tail veins in vitro (1×106/mL),the combine group were also given the decoction in oral for 10 d.BMSCs group were injected with BMSCs through tail veins(1×10 6/mL).The normal and model groups were in-jected with 1 mL of normal saline through tail veins.5 rats were killed on 10th day after the intervention,respectively,for specimen.The expression of Lgr5,Ephrin-B3 and E-cadherin were detected with Western blot,and the levels of IL-6,IL-17 and TGF-βwith ELISA assay.RESULTS The expression of Lgr5 and Ephrin-B3 in treatment groups increased(P <0.05), compared with the model group.E-cadherin in treatment groups increased compared with the model group,and the combine group and BMSCs intervene group increased significantly(P <0.05).The levels of IL-6 and IL-17 in combine group and BM-SCs group significantly decreased(P <0.05),while TGF-βincreased(P <0.05).The difference between the combine group and BMSCs group was significant(P <0.05) in Lgr5,Ephrin-B3,E-cadherin,IL-6 and TGF-βexpression.CONCLUSION Jianpi-Bushen Fang can promote the venously transplanted BMSCs proliferation and differentiation into intestinal stem cell, regulate immune function and repair intestinal mucosa,which may be one possible mechanism for UC treatment.
