The Inhibition of Medicine Composition Extracts from Keliu Pills on Human Lung Cancer Cells
10.14148/j.issn.1672-0482.2015.0555
- VernacularTitle:克瘤丸组成药物提取物对人肺癌细胞的抑制作用
- Author:
Yan FAN
1
;
Xiao-Bin CHEN
;
Rong-Li QIU
;
Li ZHANG
;
Bei-Hua BAO
;
Yong BIAN
;
Lin LI
Author Information
1. 南京中医药大学药学院
- Keywords:
Keliu Pills;
extracts;
human lung adenocarcinoma A549 cells;
MTT assay;
orthogonal experiment design
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(6):555-559
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the inhibitory effects of five medicine composition extracts from Keliu Pills on human lung adenocarcinoma A549 cells and streamline the prescription.METHODS MTT assay was used to investigate antiprolifera-tive effects of five extracts(total astragalosides,supercritical fluid extracts of cantharis,alcohol extract of insect,total alka-loids from tonkinensis and aqueous extract of astragalus and tonkinensis)on A549 cells.Five factors two levels orthogonal ex-periment design was used to determine the different antiproliferative effects of five extracts against A549 cells and to explore the interaction between each extract.RESULTS Cytotoxic experiment results showed that IC50 values of supercritical fluid ex-tracts of cantharis,alcohol extract of insect drugs,total astragalosides and total alkaloids from tonkinensis were 0.330,1.041, 1.961,1.041 mg/mL,respectively.The inhibitory rates of supercritical fluid extracts of cantharis,alcohol extract of insect drugs reached the peak at 48 h,with a concentration-effect and time-effect relationship.Orthogonal experiment showed that al-cohol extract of insect drugs played a main role in antiproliferation against A549 cells.Supercritical fluid extracts of cantharis and total astragalosides followed.Analysis of variance showed that interaction effects were found between the total astragalo-sides and alcohol extract of insect drugs,as well as supercritical fluid extracts of cantharis and alcohol extract of insect drugs. CONCLUSION The alcohol extract of insect drugs,supercritical fluid extracts of cantharis and total astragalosides are neces-sary for Keliu Pills,which can be streamlined for the compatibility of these three extracts.
