Rehmanniae Intervention of High-glucose Induced HRMC Proliferation and ECM Secretion

VernacularTitle:生地对高糖致人肾小球系膜细胞增殖、氧化应激及细胞外基质的影响
Author: Gao-Hong LYU ¹ ; Hui-Qin XU ; Xing LYU
Author Information

1. 南京中医药大学教学实验中心
Keywords: Rehmanniae; serum containing drug; HRMC; ECM; TGF-β1
From: Journal of Nanjing University of Traditional Chinese Medicine 2015;(6):551-554
CountryChina
Language:Chinese
Abstract: OBJECTIVE To investigate the effects of Rehmanniae-medicated serum on human glomerular mesangial cells (HRMC)stimulated with high concentration of glucose.METHODS Rehmanniae-medicated serum was prepared and analyzed for its serum components using LC-MS technology.MTT assay was used to detect the inhibitory rate of HRMC.Cell superna-tant levels of SOD and MDA were measured by kits.ELISA assays were used to determine the contents of fibronectin and type IV collagen.Flow cytometry was used to analyze cell cycle distributions.Immunofluorescence was used to detect the expres-sion of TGF-β1 in HRMC.RESULTS Rehmanniae-medicated serum contains catalpa alcohol and stachyose.These active com-ponents could inhibit high level glucose-induced HRMC proliferation,reduced the production of extracellular matrix,and downregulated the mRNA expression of TGF-β1 .CONCLUSION Rehmanniae could inhibit ECM production by regulating TGF-β1 so as to prevent the development of diabetic nephropathy.