Effects of Electroacupuncture at Back?shu and Front?mu Points on Expression of GDNF in Colon Tissue of Functional Constipation Mice
10.14148/j.issn.1672?0482.2015.0475
- VernacularTitle:电针大肠俞募穴对功能性便秘小鼠结肠组织 GDNF 表达的影响
- Author:
Wei ZHANG
1
;
Ying LI
;
Lisha LIU
;
Fangli LUO
;
Qianhua ZHENG
Author Information
1. 成都中医药大学针灸推拿学院
- Keywords:
electroacupuncture;
Backshu and Frontmu points;
functional constipation;
GDNF
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(5):475-479
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACTOBJECTIVE To explore the mechanism of electroacupuncture at Back?shu and Front?mu points for functional constipation treatment.METHODS 32 mice were randomly divided into control groupmodel groupelectroacupunctureEA group and EGC group.Compound diphenoxylate were gavaged for modelingand electroacupuncture Tianshu and Dachangshu points30 min each time for a total of 5 times treatment.By light microscopyelectron microscopyimmunohisto-chemistry and in situ hybridizationthe morphology of colonic epithelial structure were observedand the level of GDNF pro-tein and mRNA expression in colon were detected.RESULTS The expression of GDNF protein and mRNA in model group was significantly decreasedP <0.01.Electroacupuncture at back?Shu and front?Mu points improved the expression of GDNF protein and mRNAP <0.05and light microscope and electron microscope showed that acupuncture repaired damaged depi-thelial cellsand then improved the intestinal transmission function.The expression of GDNF protein in EGC group showed no significant difference with the model groupP >0.05but significantly decreasedcompared with EA groupP <0.05.GD-NF mRNA was significantly higher than model groupP <0.05but lower than EA group with no significant differenceP>0.05.The morphology change of colon tissue is more serious.CONCLUSION Electroacupuncture at back?Shu and front? Mu points can improve the expression of GDNF protein and mRNA in EGC cellsrepair the colonic epithelial cellsand then improve the intestinal transmission function.
