San Huang Decoction Promotes Apoptosis of Breast Cancer Cells Through Regulating Aurora Kinase A
10.14148/j.issn.1672?0482.2015.0469
- VernacularTitle:三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究
- Author:
Yanlei XU
1
;
Xiyan CHEN
;
Xu CHEN
;
Weihe BIAN
;
Chang YAO
;
Xiaoshu ZHU
;
Jiajing CHEN
Author Information
1. 南京中医药大学附属医院
- Keywords:
San Huang decoction;
Aurora A;
breast cancer cells;
apoptosis
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(5):469-474
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACTOBJECTIVE To explore the effect of San Huang decoction on the apoptosis of breast cancer cells and the effect on the mRNA and protein expression and function of Aurora kinase A and discuss the underlying mechanism of San Huang in-duced apoptosis.METHODS The inhibition of breast cancer cells proliferation was determined by CCK?8 assay.The apoptosis of breast cancer cells was detected by AnnexinV?FITC/PI Staining.The expression of mRNA of Aurora A was examined by q ?PCR analysis.The expression of apoptosis?related proteins and Aurora A were determined by Western Blot analysis.RE-SULTS San Huang decoction inhibited the proliferation of breast cancer cells in a does?dependent mannerP <0.05.The effect of inhibition caused by San Huang decoction 48 hours after delivering to breast cancer cells was better than 24 hoursP <0.05 although similar as 72 hoursP >0.05.San Huang decoction was also found to induce apoptosis in both MCF?7 and MDA?MB?231 cell lines in a dose?dependent manner.Consistent with cellular resultsSan Huang decoction treatment signifi-cantly increased the apoptosis?related protein level of cleaved?PARPc?PARPcleaved?Caspase 3c?Caspase 3and Baxdown ?regulated Bcl?2 in a does?dependent manner.MeanwhileSan Huang decoction decreased the mRNA and protein level of Auro-ra A and increased those of p53 in a does?dependent manner.CONCLUSION San Huang decoction at the first time was able to promote the apoptosis of breast cancer cells via inducing the suppression of Aurora A.
