Effect of Active Components from Lycii Cortex on Proliferation of High Glucose?induced Glomerular Mesangial Cells and Extracel-lular Matrix

VernacularTitle:地骨皮活性成分对高糖致肾小球系膜细胞增殖及细胞外基质的影响
Author: Ying ZHOU ¹ ; Wenxia PI ; Baochang CAI ; Wenwang ZHAO ; Pujun HUANG
Author Information

1. 南京中医药大学药学院
Keywords: betaine; kaempferol; glomerular mesangial cells; diabetic nephropathy; Lycii cortex
From: Journal of Nanjing University of Traditional Chinese Medicine 2015;(5):465-468
CountryChina
Language:Chinese
Abstract: ABSTRACTOBJECTIVE To observe the effect of active components from Lycii cortex on the proliferation of high glucose?in-duced glomerular mesangial cells GMCs and the secretion of extracellular matrix ECM.To investigate the protective mechanism of diabetic nephropathy.METHODS GMCs were cultured and divided into high glucose groupfinal glucose con-centration of 30 mmol/Lblank groupfinal glucose concentration of 5.5 mmol/Lbetaine and kaempferol groups of 5 dif-ferent concentrations of 0.010.1110100 μmol/L.The proliferation of high glucose?induced GMCs were observed by MTT method and secretion of ECM was detected by ELISA assay.RESULTS Different doses of betaine and kaempferol inhib-ited the proliferation of GMCsand decreased the content of ECMcompared with high glucose group P < 0.05 ~ 0.01. CONCLUSION Betaine and kaempferol can protect the high glucose?induced GMCs through inhibiting the proliferation of GMCs and decreasing the content of ECM.