Antioxidant Effects of Buxin Ruanmai Granules Serum on AngⅡ?induced Human Umbilical Vein Endothelial Cells
10.14148/j.issn.1672?0482.2015.0461
- VernacularTitle:补心软脉颗粒含药血清对 AngⅡ诱导的 HUVEC 细胞抗氧化作用
- Author:
Dong YAN
1
;
Yuliang QIAN
;
Shihai YAN
;
Ning WANG
;
Chaowei ZHOU
;
Shuhua TANG
Author Information
1. 南京中医药大学附属医院
- Keywords:
Buxin Ruanmai granules;
drug serum;
AngⅡ;
HUVEC;
oxidation inhibition
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(5):461-464
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACTOBJECTIVE To observe the effects of Buxin Ruanmai granules BXRM serum on AngiotensinⅡAngⅡ?in-duced oxidative damage related indicatorssuch as SODNADPH MDAp40phoxp47 phox and p67 phoxin HUVEC.METHODS Rats were randomly divided into normal groupValsartan group and BXRM groupeach group of 10and lavaged for 6 weeks.Drug serum were prepared from arterial blood.Then 10-6 mol/L Ang Ⅱ induced HUVEC damage and apoptosis mod-el of human umbilical vein endothelial cells was establishedand drug serum was used to intervene.SOD were determined by xanthinoxidase.NADPH was determined by ELISA.MDA was determined by thibabituric acid method.The expression of p40phoxp47phox and p67phox in cell membrane and cytoplasm of HUVEC were determined by Western blot.RESULTS After HUVECs were damaged by 10-6 mol/L Ang Ⅱthe content of SOD decreased NADPH and MDA increased and p40phoxp47phox and p67phox transferred to the membrane of HUVECs.Howeverthe content of SOD increasedNADPH and MDA de-creasedand p40phoxp47phox and p67 phox were inhibited to transfer to the membraneafter the medication of BXRM serum. CONCLUSION BXRM serum can inhibited the oxidation of AngⅡ induced HUVEC damage and prevent arteriosclerosis.
