Differential Serum Proteomic Analysis of Ankylosing Spondylitis Patients with Damp?heat Arthralgia Spasm Syndrome
10.14148/j.issn.1672?0482.2015.0412
- VernacularTitle:强直性脊柱炎湿热痹阻证血清差异蛋白组学研究
- Author:
Zhi-Ling SUN
1
;
Xiao XU
;
Ling WANG
;
Fuqiang WANG
;
Zhi-Yang XU
;
Wei JI
Author Information
1. 南京中医药大学护理学院
- Keywords:
damp-heat arthralgia spasm syndrome;
ankylosing spondylitis;
two-dimensional gel electrophoresis;
mass spec-trometry;
serum
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(5):412-415
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACTOBJECTIVE Proteomic technique was applied to analyze ankylosing spondylitis AS patients with damp?heat arthralgia spasm syndrome.METHODS Serum proteins of AS patients with damp?heat arthralgia spasm syndrome groupn=30 was compared with that of normal control group n = 30 and AS patients with cold?dampness arthralgia spasm syn-drome groupn =30 respectively by two?dimensional gel electrophoresisthe common differential protein spots of damp?heat arthralgia spasm syndrome were analyzed by the second order MS.The validation of the differential protein was performed u-sing Western blot.RESULTS Seven kinds of common differential protein spots were selected and five kinds of proteins were i-dentified by mass spectrometry.They were fibulin 1dipeptidy lpeptidase 1kininogen 1mutant IL 17Fmannose?binding protein C and related to extracellular matrix proteinproteasecoagulation factorcytokinetransport proteinand so on.Re-sults of Western blot were consistent with the proteomic results.CONCLUSION These proteins are implicated in the patho-genesis and pathological processes of AS with damp?heat arthralgia spasm syndromeand provide a basis for further studies of the essence of the syndrome in traditional Chinese medicine.
