Effect of Aristolochic Acid Ⅰ on PI3K/Akt/NF-κB Signaling Pathway in Rats
10.14148/j.issn.1672-0482.2015.0250
- VernacularTitle:马兜铃酸Ⅰ对大鼠体内 PI3K／Akt／NF-κB 通路的影响
- Author:
Zhao-Cong YANG
1
;
Yin LU
;
Ya-Qin GU
;
Zhi-Zhi QIAN
;
Si-Liang WANG
;
Zhong-Hong WEI
;
Xiao-Bo SHENG
;
Liang ZHANG
Author Information
1. 南京中医药大学药学院
- Keywords:
aristolochic acid Ⅰ;
kidney injury;
PI3K/Akt/NF-κB signaling pathway;
inflammation
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(3):250-253
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To investigate the potential mechanism of aristolochic acid Ⅰ(AAⅠ)on aristolochic acid nephrop-athy and the effect of AAⅠ on PI3K/Akt/NF-κB signaling pathway in rats.METHODS 27 male SD rats were randomly di-vided into three groups:control group,AAⅠ low-dose group(2.25 mg/kg) and AAⅠ high-dose group (9.0 mg/kg).Rats were intraperitoneally injected with AAⅠ once daily for 14 d.Then the serum were taken to determine the content of Cr,BUN and AKP in rats;renal pathological changes were examined by HE staining;the expression levels of PI3K,Akt,NF-κB were analyzed by Western blot;the expression changes of IL-6 and TNF-αwere detected by immunohistochemical assay.RESULTS Compared with control group,the levels of Cr,BUN and AKP in AAⅠ groups were increased significantly(P <0.05);kidney pathological examination revealed the improvement of kidney disease and inflammation;the expressions of IL-6 and TNF-αwere increased significantly(P <0.01);the expression of PI3K,Akt and NF-κB were up-regulated.CONCLUSION AAⅠ can induce the kidney injury and inflammation and activate the PI3K/Akt/NF-κB signaling pathway,thus worsen patho-logical changes of kidney tissue.
