Corni Fructus Extract Increased Glucose Consumption and Inhibited Isoproterenol-induced Lipolysis in 3T3-L1 Mature Adipocytes
10.14148/j.issn.1672-0482.2015.0238
- VernacularTitle:山茱萸提取物对3T3-L1脂肪细胞葡萄糖消耗与脂肪分解的影响
- Author:
Xiao-Ke LI
1
;
Dan-Dan ZHAO
;
Na YU
;
Xin FANG
;
Yi ZHANG
;
Fang-Fang MO
;
Si-Hua GAO
Author Information
1. 北京中医药大学糖尿病研究中心
- Keywords:
Corni fructus;
iridoid glycoside;
mature adipocyte;
AMPK
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(3):238-241
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To observe the effects of iridoid glycoside extract from Corni Fructus(ECO)on glucose consump-tion and isoproterenol (ISO)-induced lipolysis in 3T3-L1 mature adipocytes and possible mechanism.METHODS Glucose consumption of mature adipocytes intervened by ECO for 24 h was detected in GOP-POD method.After 2 d of ECO interven-tion,colorimetric method was used to evaluate the release amount of glycerol from 30 min ISO-induced lipolysis.The expres-sion of AMPK,GLUT4 and HSL mRNA were detected by Real-time PCR and phosphorylation levels of AMPK and ACC were evaluated by immunoblot.RESULTS In mature 3T3-L1 adipocytes,1 g/L ECO intervention increased glucose consumption and reduced the glycerol release amout from lipolysis induced by ISO with different concentrations.0.2 g/L and 0.04 g/L ECO decreased 100 nmol/L ISO-induced lipolysis.After 2 d intervention,ECO upregulated the levels of AMPK and GLUT4 mRNA and decreased the gene expression of HSL.1 g/L ECO promoted the phosphorylation of AMPK and ACC.CONCLUSION ECO can increase glucose consumption and inhibited ISO-induced lipolysis in 3T3-L1 mature adipocytes and the effects might be related to AMPK regulation.
