Protective Effects of Total Flavonoids from Bamboo Leaves on UVB-irradiated Oxidative Damage in HaCaT Cells
10.14148/j.issn.1672-0482.2015.0165
- VernacularTitle:竹叶总黄酮对UVB诱导 HaCaT细胞氧化损伤的保护作用
- Author:
Yang GAO
1
;
Ruo-Kun YI
;
Jia-Le SONG
Author Information
1. 江苏省苏北人民医院药剂科
- Keywords:
total flavonoids;
Phyllostachys pubescens leaves;
HaCaT cells;
UVB;
antioxidant
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(2):165-169
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the protective effects of total flavonoids from bamboo (Phyllostachys pubescens) leaves (BLE) on UVB-irradiated oxidative damage in HaCaT keratinocytes.METHODS The HaCaT cells were pretreated with BLE for 24 h and then exposed to UVB(20 mJ/cm2) for 2 h.Cell viability was determined by MTT assay.The levels of reactive oxygen species (ROS),lipid peroxidation and antioxidant enzymes including catalase (CAT),superoxide dismutase (SOD) and glutathione peroxidase(GSH-Px) were measured.mRNA transcription levels of these antioxidant enzymes were determined by RT-PCR assay.In addition,the production of TNF-αand IL-6 were detected by ELISA assay.RESULTS BLE significantly reduced the UVB-induced HaCaT cell damage and improved the cell survival rates (P<0.05).Compared to the control group,BLE decreased the levels of ROS and lipid peroxidation,and increased the activities and mRNA transcription levels of endogenous antioxidant enzymes(SOD,CAT and GSH-Px)in UVB-irradiated HaCaT cells.In addition,BLE also in-hibited the secretion of TNF-αand IL-6 in UVB-irradiated HaCaT cells.CONCLUSION BLE can exhibit cytoprotective activi-ties against UVB-induced oxidative damage in HaCaT cells through the reduction of ROS,inhibition of lipid peroxidation and regulation of antioxidant enzymes activities.In addition,BLE can also decrease the levels of TNF-αand IL-6 in UVB-irradiated HaCaT cells.
