The Neuroprotective Effects of Yueju Pills on Parkinson's Disease Model in vitro
10.14148/j.issn.1672-0482.2015.0156
- VernacularTitle:越鞠丸对帕金森病体外模型的神经保护作用研究
- Author:
Chang CHEN
1
;
Juan-Juan TANG
;
Bao-Mei XIA
;
Qing CHEN
;
Qi-Sheng WANG
;
Wei-Wei TAO
;
Yang ZHAO
;
Gang CHEN
Author Information
1. 南京中医药大学基础医学院
- Keywords:
Yuej u alcohol extract;
Parkinson's disease;
apoptosis;
PACAP
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2015;(2):156-159
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the neuroprotective effect and the mechanism of Yueju alcohol extract (YJ-E) on chromaffin cells(PC12)injury in rats induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine ion(MPP+).METHODS PC12 cells were given 1 mmol MPP+ to establish Parkinson's disease(PD) model in vitro.The protective effects of YJ-E on PC12 cells were measured by MTT assay and Hoechst staining.The expression of pituitary adenylate cyclase activating poly-peptide(PACAP)and the phosphorylation of extracellular regulated kinase(ERK)and cAMP response element binding pro-tein(CREB)were determined by Western blotting.RESULTS YJ-E(1.0,2.0,4.0 mg/mL)improved the survival of MPP+-injured PC12 cells significantly(P<0.01),and displayed no significant effect on normal PC12 cells(P>0.05).Meanwhile, YJ-E up-regulated the expression of PACAP and the phosphorylation of ERK and CREB significantly(P<0.01)of MPP+-in-jured PC12 cells.CONCLUSION YJ-E shows promising neuroprotective effects on PC12 cells in MPP+-induced Parkinson's disease model,and the mechanism may be associated with the up-regulation of the expression of PACAP and the phosphoryla-tion of downstream ERK and CREB.
