Effect of flow cytometry on detection of peripheral blood lymphocyte subsets in patients with acute myeloid leukemia
10.7619/jcmp.201721010
- VernacularTitle:流式细胞学技术检测急性髓细胞白血病患者外周血淋巴细胞亚群的效果分析
- Author:
Hongmei WANG
1
;
Yunning ZHANG
;
Yun LUO
Author Information
1. 江苏省泰兴市人民医院检验科
- Keywords:
flow cytometry;
acute myeloid leukemia;
peripheral blood lymphocyte subsets
- From:
Journal of Clinical Medicine in Practice
2017;21(21):36-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effect of flow cytometry on detection of peripheral blood lymphocyte subsets in patients with acute myeloid leukemia.Methods A total of 108 patients with acute myeloid leukemia were selected as leukemia group,and 108 healthy people were selected as control group.MultiSE lymphocyte subsets regents and flow cytometer (CD45/SSC,America) were used to detect 10 000 cells,and FACS Diva software was used to analyze the cellular immunophenotype and expression intensity.Results In the leukemia patients,the CD3,CD4,CD4 +/CD8 +,CD16 +/CD56 + were significandy lower than normal people,while CD8 +,CD19 + were significantly higher (P ＜ 0.05).Expression of antihelion of cell CD33,CD38 and Cyt-MPO were high,respectively accounting for 72.5％,70.4％ and 67.5％,and followed by CD15,CD64,which accounting for 38％ and 32.5％ respectively.High expressions of CD33,CD64 and Cyt-MPO all showed positive correlations (P ＜ 0.05),but was not related with expression of CD15 (P ＞ 0.05).Conclusion Flow cytometric analysis of peripheral blood lymphocyte subsets in patients with acute myeloid leukemia can provide a clear understanding of the change of lymphocyte subsets.
