Genomic Epidemiology of Carbapenemase-producing Klebsiella pneumoniae in China
- Author:
Li CUIDAN
1
;
Jiang XIAOYUAN
;
Yang TINGTING
;
Ju YINGJIAO
;
Yin ZHE
;
Yue LIYA
;
Ma GUANNAN
;
Wang XUEBING
;
Jing YING
;
Luo XINHUA
;
Li SHUANGSHUANG
;
Yang XUE
;
Chen FEI
;
Zhou DONGSHENG
Author Information
1. CAS Key Laboratory of Genome Sciences&Information,Beijing Institute of Genomics,Chinese Academy of Sciences and China National Center for Bioinformation,Beijing 100101,China
- Keywords:
Klebsiella pneumoniae;
Drug resistance;
Carbapenemase;
Plasmid;
Genomic epidemiology
- From:
Genomics, Proteomics & Bioinformatics
2022;(6):1154-1167
- CountryChina
- Language:Chinese
-
Abstract:
The rapid spread of carbapenemase-producing Klebsiella pneumoniae(cpKP)poses seri-ous threats to public health;however,the underlying genetic basis for its dissemination is still unknown.We conducted a comprehensive genomic epidemiology analysis on 420 cpKP isolates col-lected from 70 hospitals in 24 provinces/autonomous regions/municipalities of China during 2009-2017 by short-/long-read sequencing.The results showed that most cpKP isolates were categorized into clonal group 258(CG258),in which ST11 was the dominant clone.Phylogenetic analysis revealed three major clades including the top one of Clade 3 for CG258 cpKP isolates.Additionally,carbapenemase gene analysis indicated that blaKPC was dominant in the cpKP isolates,and most blaKPC genes were located in five major incompatibility(Inc)groups of blaKPC-harboring plasmids.Importantly,three advantageous combinations of host-blaKPC-carrying plasmid(Clade 3.1+3.2-IncFⅡpHN7A8,Clade 3.1+3.2-IncFⅡpHN7A8:IncR,and Clade 3.3-IncFⅡpHN7A8:InCpA1763-KPC)were identified to confer cpKP isolates the advantages in both genotypes(strong correlation/coevolution)and phenotypes(resistance/growth/competition)to facilitate the nationwide spread of ST11/CG258 cpKP.Intriguingly,Bayesian skyline analysis illustrated that the three advanta-geous combinations might be directly associated with the strong population expansion during 2007-2008 and subsequent maintenance of the population of ST11/CG258 cpKP after 2008.We then examined drug resistance profiles of these cpKP isolates and proposed combination treatment regimens for CG258/non-CG258 cpKP infections.Thus,the findings of our systematical analysis shed light on the molecular epidemiology and genetic basis for the dissemination of ST11/CG258 cpKP in China,and much emphasis should be given to the close monitoring of advantageous cpKP-plasmid combinations.
