Effect of siRNA silencing epidermal growth factor receptor protein expression on biological behavior of renal cell carcinoma cells
10.7619/jcmp.201719029
- VernacularTitle:siRNA沉默表皮生长因子受体蛋白表达对肾癌细胞生物学行为的影响
- Author:
Feng WANG
1
;
Binbin ZHANG
;
Wei GUO
;
Jixue GAO
;
Yayong QIANG
;
Junqi JIA
Author Information
1. 延安大学附属医院,陕西延安,716000
- Keywords:
siRNA;
EGFR protein;
renal cell carcinoma;
biological behavior
- From:
Journal of Clinical Medicine in Practice
2017;21(19):103-106
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological behavior of renal cell carcinoma cases after treatment with siRNA silencing epidermal growth factor receptor (EGFR) gene.Methods Renal cell carcinoma cell line ACHN were divided into three groups,the control group without any intervention,negative control transfection group by adding the nonspecific transfection reagent and siRNA interference,and the observation group adding transfection reagent and siRNA interference.Cells with EGFR expression and proliferation activity,growth curve,migration and invasion activity were detected in three groups.Results After 72 h of siRNA treatment,the observation group had significantly lower EGFR expression levels,and cell growth activity ability,and cell number of RNAi group after 48 h than negative control transfection group and control group (P ＜ 0.05).Compared with the blank control group and negative control transfection group,the observation group was significantly inhibited (P ＜0.05),and cell scratch distance after 12 h and 24 h was significantly higher than that of the control group and negative control transfection group (P ＜ 0.05).The control group and negative control transfection group showed no significant difference in the cell membrane at 12 h (P ＞0.05).The observation group had significantly lower transmembrane cell number than the control group and negative control transfection group (P ＜ 0.05).Conclusion The proliferation,growth,migration and invasion of renal carcinoma cells can be effectively improved by using siRNA EGFR protein.
