Clinical value of human papillomavirus nucleic acid detection kit in screening cervical cancer and precancerous lesions
10.7619/jcmp.201719028
- VernacularTitle:人乳头状瘤病毒核酸检测试剂盒用于宫颈癌及癌前病变筛查的临床价值
- Author:
Lele SHANG
1
;
Jiefeng HU
;
Yueqing WANG
;
Yinyin ZHU
;
Shougang KUAI
Author Information
1. 江苏省无锡市惠山区人民医院
- Keywords:
human papillomavirus;
human papillomavirus;
cervical cancer;
screening
- From:
Journal of Clinical Medicine in Practice
2017;21(19):99-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the clinical value of the second generation hybrid capture nucleic acid detection kit (DH3) of human papillomavirus (HPV) in screening cervical cancer and precancerous lesions.Methods The cervical exfoliated cell samples of 480 women in gynecologic clinic were collected and detected by DH3,TCT and HPV-PCR.Colposcopic pathological examination was performed in women with TCT ≥ ASC-US.With the pathological results as the golden standard,the diagnostic value of DH3 was analyzed.Results The pathological results showed that 370 cases were normal (77.08％),59 cases had benign lesions (≤CIN Ⅰ) (12.29％) and 51 were high-risk cases (≥CIN Ⅱ) (10.63％).The positive diagnostic rates of TCT,HPV-PCR and DH3 were 26.04％,32.08％ and 27.08％,respectively.The concordancerate of DH3 and TCT was 94.79％ (P ＜ 0.01),and the concordance rate with HPV-PCR was 93.13％ (P ＜ 0.01).The sensitivity,specificity,positive predictive value,negative predictive value and accuracy rate of DH3 were 98.18％,87.57％,70.13％,99.39％ and 90％,respectively.The ROC area of detection of high-risk (CIN Ⅱ) was Z =0.887 (95 ％ CI 0.785 ～ 0.918,P ＜ 0.01).Conclusion DH3 kit has a high degree of consistency with TCT and HPV-PCR in the detection of HPV cervical cancer and precancerous lesions.The sensitivity,specificity and accuracy are high,and it is easy to operate.
