The role of microRNA-192 in progression of advanced glycation end products-induced epithelial-to-mesenchymal transition of human peritoneal mesothelial cells
10.7619/jcmp.201719027
- VernacularTitle:microRNA-192在晚期糖基化终产物诱导人腹膜间皮细胞上皮-间叶转化中的作用
- Author:
Weibin SHAO
1
;
Ping LU
;
Chunying XIA
;
Xin LI
;
Kang XUN
;
Songtao FENG
;
Qian ZHAO
Author Information
1. 江苏省镇江市第一人民医院肾脏内科
- Keywords:
peritoneal mesothelial cells;
epithelial-to-mesenchymal transition;
advanced glycation end products;
microRNA-192
- From:
Journal of Clinical Medicine in Practice
2017;21(19):95-98
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of microRNA-192 (miR-192) in the regulation of epithelial mesenchymal transition induced by advanced glycation end products (AGEs) in human peritoneal mesothelial cells.Methods The human peritoneal mesothelial cells,the human peritoneal mesothelial cells transfected by miR-192 inhibitor and the human peritoneal mesothelial cells transfected by miR-192 inhibitor negative control were cultured for 72 hours in culture medium containing AGEs (80mM);M199 medium and medium with M199 medium containing 80mM BSA as negative control,the expression of miR-192 and mRNA was detected by real-time quantitative PCR,and the expression of miR-192 and mRNA was detected by Western blotting.Results AGEs significantly upregulated the expression of miR-192,Collagen Ⅰ mRNA,α-smooth muscle actin (α-SMA) mRNA and protein(P ＜ 0.05),while significantly downregulated the E-cadherin mRNA and protein expression(P ＜ 0.05).Compared with human peritoneal mesothelial cells transfected by miR-192 inhibitor,the expression of miR-192 and Collagen Ⅰ mRNA and α-SMA mRNA and protein was decreased (P ＜0.05),while E-cadherin mRNA and protein expression was significantly increased (P ＜ 0.05).Conclusion AGEs may induce EMT of human peritoneal mesothelial cells by upregulation of miR-192 expression.MiR-192 inhibitor may prevent AGEs-inducing EMT of human peritoneal mesothelial cells by down-regulation of miR-1 9 2 expression,and may play an important regulatory role in EMT of human peritoneal mesothelial cells induced by AGEs.
