Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium
- Author:
Jin XIUXIU
1
,
2
,
3
;
Liu JINGYANG
;
Wang WEIPING
;
Li JIANGFENG
;
Liu GUANGMING
;
Qiu RUIQI
;
Yang MINGZHU
;
Liu MENG
;
Yang LIN
;
Du XIAOFENG
;
Lei BO
Author Information
1. Henan Eye Institute,Henan Eye Hospital,People's Hospital of Zhengzhou University,Henan Provincial People's Hospital,Zhengzhou 450003,China
2. Branch of National Clinical Research Center for Ocular Disease,Henan Provincial People's Hospital,Zhengzhou 450003,China
3. School of Medicine,Henan Provincial People's Hospital,Henan University,Zhengzhou 450003,China
- Keywords:
Human retinal pigment epithelium;
Proteomics;
Aging;
Retina;
Apoptosis
- From:
Genomics, Proteomics & Bioinformatics
2022;20(4):633-647
- CountryChina
- Language:Chinese
-
Abstract:
Retinal pigment epithelium(RPE)has essential functions,such as nourishing and sup-porting the neural retina,and is of vital importance in the pathogenesis of age-related retinal degen-eration.However,the exact molecular changes of RPE during aging remain poorly understood.Here,we isolated human primary RPE(hRPE)cells from 18 eye donors distributed over a wide age range(10-67 years old).A quantitative proteomic analysis was performed to analyze changes in their intracellular and secreted proteins.Age-group related subtypes and age-associated proteins were revealed and potential age-associated mechanisms were validated in ARPE-19 and hRPE cells.The results of proteomic data analysis and verifications suggest that RNF123-and RNF149-related protein ubiquitination plays an important role in protecting hRPE cells from oxidative damage dur-ing aging.In older hRPE cells,apoptotic signaling-related pathways were up-regulated,and endo-plasmic reticulum organization was down-regulated both in the intracellular and secreted proteomes.Our work paints a detailed molecular picture of hRPE cells during the aging process and provides new insights into the molecular characteristics of RPE during aging and under other related clinical retinal conditions.
