DEBKS:A Tool to Detect Differentially Expressed Circular RNAs
- Author:
Liu ZELIN
1
;
Ding HUIRU
;
She JIANQI
;
Chen CHUNHUA
;
Zhang WEIGUANG
;
Yang ENCE
Author Information
1. Institute of Systems Biomedicine,Department of Medical Bioinformatics,School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100191,China
- Keywords:
RNA-seq;
Gene expression;
Circular RNA;
Back-splicing;
Ribo-Zero
- From:
Genomics, Proteomics & Bioinformatics
2022;20(3):549-556
- CountryChina
- Language:Chinese
-
Abstract:
Circular RNAs(circRNAs)are involved in various biological processes and disease pathogenesis.However,only a small number of functional circRNAs have been identified among hundreds of thousands of circRNA species,partly because most current methods are based on cir-cular junction counts and overlook the fact that a circRNA is formed from the host gene by back-splicing(BS).To distinguish the expression difference originating from BS or the host gene,we pre-sent differentially expressed back-splicing(DEBKS),a software program to streamline the discov-ery of differential BS events between two rRNA-depleted RNA sequencing(RNA-seq)sample groups.By applying to real and simulated data and employing RT-qPCR for validation,we demon-strate that DEBKS is efficient and accurate in detecting circRNAs with differential BS events between paired and unpaired sample groups.
