Integrative Proteomic Analysis of Multiple Posttranslational Modifications in Inflammatory Response
- Author:
Ji FEIYANG
1
;
Zhou MENGHAO
;
Zhu HUIHUI
;
Jiang ZHENGYI
;
Li QIRUI
;
Ouyang XIAOXI
;
Lv YIMING
;
Zhang SAINAN
;
Wu TIAN
;
Li LANJUAN
Author Information
1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,National Clinical Research Center for Infectious Diseases,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China
- Keywords:
Proteome;
Crosstalk;
Inflammation;
Lipopolysaccharide;
Macrophage
- From:
Genomics, Proteomics & Bioinformatics
2022;20(1):163-176
- CountryChina
- Language:Chinese
-
Abstract:
Posttranslational modifications(PTMs)of proteins,particularly acetylation,phosphory-lation,and ubiquitination,play critical roles in the host innate immune response.PTMs'dynamic changes and the crosstalk among them are complicated.To build a comprehensive dynamic net-work of inflammation-related proteins,we integrated data from the whole-cell proteome(WCP),acetylome,phosphoproteome,and ubiquitinome of human and mouse macrophages.Our datasets of acetylation,phosphorylation,and ubiquitination sites helped identify PTM crosstalk within and across proteins involved in the inflammatory response.Stimulation of macrophages by lipopolysac-charide(LPS)resulted in both degradative and non-degradative ubiquitination.Moreover,this study contributes to the interpretation of the roles of known inflammatory molecules and the dis-covery of novel inflammatory proteins.
