A non-targeted metabolomics study on urine of occupational exposure people with titanium dioxide nanoparticles
10.3760/cma.j.cn121094-20200323-00149
- VernacularTitle:纳米二氧化钛颗粒职业接触人群尿液非靶向代谢组学研究
- Author:
Xiaodong LIU
1
;
Chao WANG
;
Xiangjuan MENG
;
Xingfu PAN
;
Jue LI
;
Dongsheng NIU
;
Zhangjian CHEN
Author Information
1. 100093 北京市职业病防治研究院
- Keywords:
Occupational exposure;
Metabolomics;
Urine;
Titanium dioxide nanoparticles;
Ultra-high performance liquid chromatography time-of-flight mass spectrometry
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(5):328-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of titanium dioxide nanoparticles (TiO 2 NPs) on urine metabolites in occupationally exposure people based on metabolomics technology, and to explore the mechanism of early health effects of TiO 2 NPs on occupational exposure. Methods:In October 2019, the TiO 2 NPs occupational exposure population was selected as the research object, of which 64 people were in the exposure group who had been engaged in TiO 2 NPs exposure positions for more than 1 year; the control group was 62 people, who were logistics administrative staff of the same company. The urine of the research subjects before class was collected, using the ultra-high performance liquid chromatography time-of-flight mass spectrometer to collect the metabolism data of the urine, Progenesis QI software for data preprocessing and metabolite identification, SIMCA-P software for the principal component analysis of the data and potential biomarkers screening, MetaboAnalyst 4.0 software for metabolic pathway enrichment analysis. Results:The urine metabolism profile of workers in the exposure group was different from the control group, and 44 potential biomarkers were screened and identified. These potential biomarkers were significantly enriched in three pathways ( P<0.05) , namely D-arginine and D-ornithine metabolism pathway, nitrogen metabolism pathway and D-glutamine and D-glutamate metabolism pathways. Conclusion:The occupational exposure of TiO 2 NPs can affect the concentration of metabolites in people urine and metabolic pathways, which provides a direction for the study of occupational hazard mechanisms of TiO 2 NPs and the monitoring of health risks.
