Effect of yiqi yangyin tongluo regime on expression of TNF-α in renal tissue in rats with IgA nephropathy
- VernacularTitle:益气养阴通络方对IgA肾病模型大鼠肾脏组织肿瘤坏死因子-α的影响
- Author:
Sheng QIANG
1
;
Zhenfang DU
;
Min HUANG
;
Xiaowei MA
;
Yicen ZHOU
Author Information
- Keywords: IgA nephropathy; yiqi yangyin tongluo regime; mechanism; TNF-α
- From: Journal of Clinical Medicine in Practice 2017;21(17):64-68
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the effect of yiqi yangyin tongluo regime on hematuria,proteinuria and renal tissue's pathological changes in rats with IgA nephropathy (IgAN).Methods Sixty clean male SD rats were randomized into blank group,model group,huangkui group and high,medium and low-dose TCM (yiqi yangyin tongluo regime) group.After modeling for 0 week,4 weeks,8 weeks,12 weeks and 16 weeks,the levels of urine red blood cell count and 24 h urine protein were detected.Meanwhile,after feeding for 8 weeks,the pathological changes of remnant renal tissues and expression of TNF-α were detected by HE staining and immunohistochemical staining.Results After feeding for 8 weeks,compared with blank group,the level of urine red blood cell count,24h urine protein and TNF-α expression in model group,huangkui group,high,medium and low-dose TCM group were significantly higher (P ＜ 0.05).Compared with the model group,the level of urine red blood cell count,24h urine protein and TNF-α expression in huangkui group,high,medium and low-dose TCM group were significantly lower (P ＜ 0.05).The above indexes in TCM group were significantly lower than huangkui group (P ＜ 0.05).Light microscopy showed that the renal tissues were normal in blank group.In the model group,most of glomerular mesangial cells and matrix appeared moderate and severe hyperplasia,inflammatory ceils and focal fibrosis were seed in renal interstitium.Proliferation of mesangial cells and matrix were relieved in huangkui group and TCM group.Conclusion Yiqi yangyin tongluo regime can effectively ameliorate the levels of urine red blood cell count,24 h urine protein and renal pathological changes.The mechanism may be related to the decrease of urine red blood cell count and 24 h urinary protein,and inhibition of TNF-α expression.
