Integrating Genomic and Transcriptomic Data to Reveal Genetic Mechanisms Underlying Piao Chicken Rumpless Trait
- Author:
Wang YUN-MEI
1
,
2
,
3
;
Khederzadeh SABER
;
Li SHI-RONG
;
Otecko Otieno NEWTON
;
M.Irwin DAVID
;
Thakur MUKESH
;
Ren XIAO-DIE
;
Wang MING-SHAN
;
Wu DONG-DONG
;
Zhang YA-PING
Author Information
1. State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,China
2. Kunming College of Life Science,University of the Chinese Academy of Sciences,Kunming 650223,China
3. Center for Neurobiology and Brain Restoration,Skolkovo Institute of Science and Technology,Moscow 143026,Russia
- Keywords:
Comparative transcrip-tomics;
Population genomics;
Rumplessness;
Vertebra development;
Artificial selection
- From:
Genomics, Proteomics & Bioinformatics
2021;19(5):787-799
- CountryChina
- Language:Chinese
-
Abstract:
Piao chicken,a rare Chinese native poultry breed,lacks primary tail structures,such as pygostyle,caudal vertebra,uropygial gland,and tail feathers.So far,the molecular mechanisms underlying tail absence in this breed remain unclear.In this study,we comprehensively employed comparative transcriptomic and genomic analyses to unravel potential genetic underpinnings of rum-plessness in Piao chicken.Our results reveal many biological factors involved in tail development and several genomic regions under strong positive selection in this breed.These regions contain can-didate genes associated with rumplessness,including Irx4,Il18,Hspb2,and Cryab.Retrieval of quantitative trait loci(QTL)and gene functions implies that rumplessness might be consciously or unconsciously selected along with the high-yield traits in Piao chicken.We hypothesize that strong selection pressures on regulatory elements might lead to changes in gene activity in mes-enchymal stem cells of the tail bud.The ectopic activity could eventually result in tail truncation by impeding differentiation and proliferation of the stem cells.Our study provides fundamental insights into early initiation and genetic basis of the rumpless phenotype in Piao chicken.
