Effects of chronic stress during pregnancy on composition and diversity of intestinal microbiota in female rats and offspring
10.3760/cma.j.cn.121094-20200310-00121
- VernacularTitle:孕期慢性应激对雌鼠和子代肠道菌群组成及多样性的影响
- Author:
Feng ZHAO
1
;
Suzhen GUAN
;
Kai WANG
;
Youjuan FU
;
Hongya LIU
;
Xiaohui CHEN
;
Faqiu QI
;
Zhihong LIU
Author Information
1. 750004 银川，宁夏医科大学公共卫生与管理学院，宁夏环境因素与慢性病控制重点实验室
- Keywords:
Rats;
Gut microbiota;
Chronic stress during pregnancy;
Female rat;
Offspring;
Community structure
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(3):161-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of chronic stress of pregnant rats on the gut microbiota of female rats and offspring, and explore the role of intestinal microbiota in chronic stress during pregnancy.Methods:In November 2019, SPF-grade healthy adult SD rats were selected. 16 female rats were randomly divided into control group and model group, with 8 in each group; 12 male rats were randomly divided into model mating group (8) and control mating group (4) . A model of chronic unpredictable mild stress (CUMS) during pregnancy was established. Blood samples were collected from the iliac vein of the female rats 1 day before and 1, 7, and 14 days after the CUMS protocol, and measured for plasma corticosterone content by radioimmunoassay. After the stress was completed, fresh feces of the female rats were collected for testing. The offspring's fresh stool samples were collected on postnatal day 20 (PND20) , and they were divided into control offspring group and model offspring group samples. The sequence of 16S rRNAV3-V4 regions of microorganisms in the feces of offspring was determined by Illumina MiSeq technique; and the interaction between microbial community structure and diversity were analyzed.Results:The content of plasma corticosterone in the model group was higher than that in the control group on the 7th and 14th day of stress ( P<0.05) . Compared with the control group, the Sobs index, Chao index, ACE index and Shannon index of the model group were decreased ( P<0.05) . The number of unique species abundance (OTU) in the control group was 130, and 91 in the model group. The relative abundance of female Firmicutes in the control group (64.87%) was higher than that in the model group, and the relative abundance of Bacteroides (31.72%) was lower than that of the model group (46.35%) . The Sobs index, Chao index, ACE index, Simpson index and Shannon index of the control offspring group were higher than those of the model offspring group ( P<0.05) . The number of unique OTUs in the model offspring group was 75, and 93 in the control offspring group. The relative abundance of Firmicutes (60.24%) in the control offspring group was higher than that of the model offspring group (52.95%) . Conclusion:Chronic stress during pregnancy can not only lead to the disorder of intestinal flora in female rats, but also lead to the change of intrauterine environment, thus affecting the diversity of intestinal flora in offspring.
