Determination of n-butyl alcohol in urine by headspace solid-phase microextraction coupled with gas chromatography
10.3760/cma.j.cn121094-20200102-00003
- VernacularTitle:尿中正丁醇测定的顶空固相微萃取-气相色谱法
- Author:
Haichuan CHEN
1
;
Sujuan PENG
;
Hongmin GAO
;
Wanting SU
;
Yong MEI
;
Guilin YI
Author Information
1. 430065 武汉科技大学医学院公共卫生学院职业危害识别与控制湖北省重点实验室
- Keywords:
Solid phase microextraction;
N-butyl alcohol;
Urine;
Chromatography, gas
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(12):932-935
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a headspace solid phase microextraction-gas chromatography method for determination of n-Butyl alcohol in urine.Methods:In October 2019, the n-butyl alcohol in urine was extracted with a polydimethylsiloxane/divinylbenzene (PDMS/DVB) solid-phase microextraction head. The conditions of salt amount, extraction temperature, extraction time and desorption time were optimized. The separation was performed on HP-5 (30 m×0.32 mm×0.25 μm) capillary column and detected with flame ionization detector. The quantification was based on the external standard curve.Results:The linear relationship of n-butyl alcohol in urine was good in the range of 0.04-3.00 mg/L, the correlation coefficient was 0.999, the detection limit of the method was 0.04 mg/L, the recovery was 77.4%-102.8%, the intra-run precision was 3.67%-8.11%, and the inter-assay precision was 4.94%-6.90%.Conclusion:The method has simple operation, high concentration efficiency and high sensitivity, and it is suitable for the determination of n-butyl alcohol in urine of occupational exposure to n-butyl alcohol.
