Landscape and Dynamics of the Transcriptional Regulatory Network During Natural Killer Cell Differentiation
- Author:
Li KUN
1
;
Wu YANG
;
Li YOUNG
;
Yu QIAONI
;
Tian ZHIGANG
;
Wei HAIMING
;
Qu KUN
Author Information
1. Department of Oncology,The First Affiliated Hospital of USTC,Division of Molecular Medicine,Hefei National Laboratory for Physical Sciences at Microscale,School of Basic Medical Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230021,China
- Keywords:
NK cell;
ATAC-seq;
Programmed differentiation;
FOSL2;
EGR2;
Dynamic regulatory network
- From:
Genomics, Proteomics & Bioinformatics
2020;18(5):501-515
- CountryChina
- Language:Chinese
-
Abstract:
Natural killer (NK) cells are essential in controlling cancer and infection. However, little is known about the dynamics of the transcriptional regulatory machinery during NK cell differen-tiation. In this study, we applied the assay of transposase accessible chromatin with sequencing (ATAC-seq) technique in a home-developed in vitro NK cell differentiation system. Analysis of ATAC-seq data illustrated two distinct transcription factor (TF) clusters that dynamically regulate NK cell differentiation. Moreover, two TFs from the second cluster, FOS-like 2 (FOSL2) and early growth response 2 (EGR2), were identified as novel essential TFs that control NK cell maturation and function. Knocking down either of these two TFs significantly impacted NK cell differentia-tion. Finally, we constructed a genome-wide transcriptional regulatory network that provides a bet-ter understanding of the regulatory dynamics during NK cell differentiation.
