The Wolfiporia cocos Genome and Transcriptome Shed Light on the Formation of Its Edible and Medicinal Sclerotium
- Author:
Luo HONGMEI
1
;
Qian JUN
;
Xu ZHICHAO
;
Liu WANJING
;
Xu LEI
;
Li YING
;
Xu JIANG
;
Zhang JIANHONG
;
Xu XIAOLAN
;
Liu CHANG
;
He LIU
;
Li JIANQIN
;
Sun CHAO
;
Martin FRANCIS
;
Song JINGYUAN
;
Chen SHILIN
Author Information
1. Engineering Research Center of Chinese Medicine Resource,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China
- Keywords:
Wolfiporia cocos;
Genome sequencing;
Sclerotial formation;
Fungal development;
Polysaccharide and triter-penoid biosynthesis
- From:
Genomics, Proteomics & Bioinformatics
2020;18(4):455-467
- CountryChina
- Language:Chinese
-
Abstract:
Wolfiporia cocos (F. A. Wolf) has been praised as a food delicacy and medicine for cen-turies in China. Here, we present the genome and transcriptome of the Chinese strain CGMCC5.78 of W. cocos. High-confidence functional prediction was made for 9277 genes among the 10,908 total predicted gene models in the W. cocos genome. Up to 2838 differentially expressed genes (DEGs) were identified to be related to sclerotial development by comparing the transcriptomes of mycelial and sclerotial tissues. These DEGs are involved in mating processes, differentiation of fruiting body tissues, and metabolic pathways. A number of genes encoding enzymes and regulatory factors related to polysaccharide and triterpenoid production were strikingly regulated. A potential triter-penoid gene cluster including the signature lanosterol synthase (LSS) gene and its modified compo-nents were annotated. In addition, five nonribosomal peptide synthase (NRPS)-like gene clusters, eight polyketide synthase (PKS) gene clusters, and 15 terpene gene clusters were discovered in the genome. The differential expression of the velevt family proteins, transcription factors,carbohydrate-active enzymes, and signaling components indicated their essential roles in the regu-lation of fungal development and secondary metabolism in W. cocos. These genomic and transcrip-tomic resources will be valuable for further investigations of the molecular mechanisms controlling sclerotial formation and for its improved medicinal applications.
