Expression of Bcl-2 and Bax proteins in the neurons of spinal cord segment after tibial nerve and common peroneal nerve injuries in rats
10.7619/jcmp.201715002
- VernacularTitle:胫神经和腓总神经损伤修复后相应脊髓节段神经元细胞Bcl-2、Bax蛋白表达差异的实验研究
- Author:
Guangxia SUN
1
;
Yaofa LIN
;
Qiang ZHANG
;
Haiyang ZONG
;
Jun ZHOU
;
Zheng XIE
;
Wanwan SHAO
;
Haodong LIN
;
Chunlin HOU
Author Information
1. 上海长征医院医疗科
- Keywords:
tibial nerve;
common peroneal nerve;
nerve injury;
nerve repairmen;
Bcl-2;
Bax
- From:
Journal of Clinical Medicine in Practice
2017;21(15):5-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expressions of Bcl-2 and Bax proteins in the neurons of spinal cord after tibial nerve and common peroneal nerve injuries in rats.Methods A total of 90 male SD rats were randomly divided into three groups, the group A was as control group, group B as suture of tibial nerve cut group, and group C as suture of the common peroneal nerve cut group.The L4-6 segment spinal cord of the rats were removed for HE staining and immumohistochemical staining on 3, 7, 14, 28 days after the operation.The number of motor neurons in the spinal anterior horn were calculated.The expressions differences of Bcl-2 and Bax proteins were detected by immuno-histochemistry, and values of Bcl-2/Bax were calculated.Results The number of motor neurons in the anterior horn of spinal cord in group B were less than that in group C on 1, 3, 7, 14, 28 days after the operation (P< 0.01).The expression of Bcl-2 protein in group B and group C showed an upward and then declining trend.It decreased to the lowest on the postoperative 3 d in group B and 7 d in group C, respectively.The expressions of Bax protein in group B and C showed an upward and then declining trend, and it increased to the highest on the postoperative 3 d in groups B and C.The values of Bcl-2/Bax in groups B and C were showed an decreased and then increased trend and it was lower in group B than group C on the postoperative 3, 7, 14, 28 d(P< 0.01).Conclusion Injuries of tibial nerve and common peroneal nerve of the rats would lead the apoptosis of motor neurons in the spinal cord, but compared with the injury of common peroneal nerve, tibial nerve injury would lead less apoptosis of motor neurons.
