The mRNA Binding Proteome of Proliferating and Differentiated Muscle Cells
- Author:
Hiller MONIKA
1
;
Geissler MIRIAM
;
Janssen GEORGE
;
Veelen Van PETER
;
Aartsma-Rus ANNEMIEKE
;
Spitali PIETRO
Author Information
1. Department of Human Genetics,Leiden University Medical Center,Leiden 2333 ZC,the Netherlands
- Keywords:
RNA binding protein;
Interactome;
Duchenne muscular dystrophy;
Proteomics;
Skeletal muscle
- From:
Genomics, Proteomics & Bioinformatics
2020;18(4):384-396
- CountryChina
- Language:Chinese
-
Abstract:
Muscle formation is a coordinated process driven by extensive gene expression changes where single cells fuse together to form multinucleated muscle fibers. Newly synthesized mRNAs are then regulated by RNA binding proteins (RBPs), affecting post-transcriptional transcript meta-bolism. Here, we determined how large-scale gene expression changes affect the catalog of RBPs by studying proliferating and differentiated muscle cells in healthy and dystrophic conditions. Tran-scriptomic analysis showed that the expression of more than 7000 genes was affected during myo-genesis. We identified 769 RBPs, of which 294 were muscle-specific and 49 were uniquely shared with cardiomyocytes. A subset of 32 RBPs (half of which were muscle-specific) was found to be preferentially associated with target mRNAs in either myoblasts (MBs) or myotubes (MTs). A large proportion of catalytic proteins were bound to mRNAs even though they lack classical RNA bind-ing domains. Finally, we showed how the identification of cell-specific RBPs enabled the identifica-tion of biomarkers that can separate healthy individuals from dystrophic patients. Our data show how interactome data can shed light on new basic RNA biology as well as provide cell-specific data that can be used for diagnostic purposes.
