G9a/GLP-sensitivity of H3K9me2 Demarcates Two Types of Genomic Compartments
- Author:
Yan ZIXIANG
1
;
Ji LUZHANG
;
Huo XIANGRU
;
Wang QIANFENG
;
Zhang YUWEN
;
Wen BO
Author Information
1. MOE Key Laboratory of Metabolism and Molecular Medicine,Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,and Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China
- Keywords:
H3K9me2;
G9a/GLP;
Chromatin organization;
3D genome;
Genomic compartment
- From:
Genomics, Proteomics & Bioinformatics
2020;18(4):359-370
- CountryChina
- Language:Chinese
-
Abstract:
In the nucleus, chromatin is folded into hierarchical architecture that is tightly linked to various nuclear functions. However, the underlying molecular mechanisms that confer these archi-tectures remain incompletely understood. Here, we investigated the functional roles of H3 lysine 9 dimethylation (H3K9me2), one of the abundant histone modifications, in three-dimensional (3D) genome organization. Unlike in mouse embryonic stem cells, inhibition of methyltransferases G9a and GLP in differentiated cells eliminated H3K9me2 predominantly at A-type (active) genomic compartments, and the level of residual H3K9me2 modifications was strongly associated with B-type (inactive) genomic compartments. Furthermore, chemical inhibition of G9a/GLP in mouse hepatocytes led to decreased chromatin-nuclear lamina interactions mainly at G9a/GLP-sensitive regions, increased degree of genomic compartmentalization, and up-regulation of hundreds of genes that were associated with alterations of the 3D chromatin. Collectively, our data demonstrated essential roles of H3K9me2 in 3D genome organization.
