Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics
- Author:
Suo JINWEI
1
,
2
,
3
;
Zhang HENG
;
Zhao QI
;
Zhang NAN
;
Zhang YONGXUE
;
Li YING
;
Song BAOHUA
;
Yu JUANJUAN
;
Cao JIANGUO
;
Wang TAI
;
Luo JI
;
Guo LIHAI
;
Ma JUN
;
Zhang XUMIN
;
She YIMIN
;
Peng LIANWEI
;
Ma WEIMIN
;
Guo SIYI
;
Miao YUCHEN
;
Chen SIXUE
;
Qin ZHI
;
Dai SHAOJUN
Author Information
1. Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University,Shanghai 200234,China
2. Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field,Ministry of Education,Harbin 150040,China
3. State Key Laboratory of Subtropical Silviculture,Zhejiang A&F U
- Keywords:
Chloroplasts;
Na2CO3 stress;
ROS scavenging;
Phosphoproteomics;
Puccinellia tenuiflora
- From:
Genomics, Proteomics & Bioinformatics
2020;18(3):271-288
- CountryChina
- Language:Chinese
-
Abstract:
Alkali-salinity exerts severe osmotic, ionic, and high-pH stresses to plants. To under-stand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species (ROS) homeostasis, physiological and diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuiflora) under Na2CO3 stress were conducted. In addition, Western blot,real-time PCR, and transgenic techniques were applied to validate the proteomic results and test the functions of the Na2CO3-responsive proteins. A total of 104 and 102 Na2CO3-responsive proteins were identified in leaves and chloroplasts, respectively. In addition, 84 Na2CO3-responsive phospho-proteins were identified, including 56 new phosphorylation sites in 56 phosphoproteins from chloro-plasts, which are crucial for the regulation of photosynthesis, ion transport, signal transduction, and energy homeostasis. A full-length PtFBA encoding an alkaligrass chloroplastic fructose-bisphosphate aldolase (FBA) was overexpressed in wild-type cells of cyanobacterium Synechocystis sp. Strain PCC 6803, leading to enhanced Na2CO3 tolerance. All these results indicate that thermal dissipation, state transition, cyclic electron transport, photorespiration, repair of pho-tosystem (PS) Ⅱ, PSI activity, and ROS homeostasis were altered in response to Na2CO3 stress, which help to improve our understanding of the Na2CO3-responsive mechanisms in halophytes.
