Determination of nickel in urine by ultrasonic-assisted ionic liquid microextraction-graphite furnace atomic absorption spectrometry
10.3760/cma.j.cn121094-20191010-00483
- VernacularTitle:尿镍测定的超声辅助离子液体微萃取-石墨炉原子吸收光谱法
- Author:
Yumeng REN
1
;
Yong MEI
;
Ruodan FANG
Author Information
1. 430065 武汉科技大学医学院公共卫生学院，武汉科技大学职业危害识别与控制湖北省重点实验室
- Keywords:
Nickel;
Urine;
Ionic liquids;
Ultrasound assisted;
Graphite furnace atomic absorption
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(10):767-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To set up a new method to determine the nickel of urine in urine using dispersive liquid-liquid microextraction (DLLME) coupled with graphite furnace atomic absorption spectrometry (GFAAS) .Methods:From September 2018 to September 2019, the methanol, pyrrolidine dithiocarbamate and ionic liquid 1-hexyl-3-methyl-imidazolium hexafluorophosphate were used as dispersive solvent, the chelating agent and extraction solvent for the preconcentration of nickel, respectively. After adding into buffer solution of pH 9, ultrasonic dissolving for 10 minutes, centrifugal separation and then discarding the supernatant, the precipitate was saved. Dissolving the precipitate by methanol, mixing thoroughly on a vortex mixer, the 15 μl of the mixed solution was used for determination by graphite furnace atomic absorption spectrometry.Results:The linear correlation coefficient of urine nickel concentration in the range of 2.0-10.0 μg/L, r=0.999, with the detection limitation of 0.43 μg/L. The recovery rate and the relative standard deviations were 95.6% -103.7% and 2.53% -4.82%, respectively. Conclusion:The method, which has low detection limit, high recovery rate and good precision, is suitable for the determination of nickel in urine for the occupational populations exposure to nickel and non-occupational exposure.
