Large-scale Identification and Time-courseQuantification of Ubiquitylation Events During Maize Seedling De-etiolation
- Author:
Wang YUE-FENG
1
;
Chao QING
;
Li ZHE
;
Lu TIAN-CONG
;
Zheng HAI-YAN
;
Zhao CAI-FENG
;
Shen ZHUO
;
Li XIAO-HUI
;
Wang BAI-CHEN
Author Information
1. Photosynthesis Research Center
- Keywords:
Maize seedling leaf;
De-etiolation;
Ubiquitylation;
C4 photosynthetic enzymes;
Ribosome
- From:
Genomics, Proteomics & Bioinformatics
2019;17(6):603-622
- CountryChina
- Language:Chinese
-
Abstract:
The ubiquitin system is crucial for the development and fitness of higher plants. De-etiolation, during which green plants initiate photomorphogenesis and establish autotrophy, is a dramatic and complicated process that is tightly regulated by a massive number of ubiquitylation/de-ubiquitylation events. Here we present site-specific quantitative proteomic data for theubiquitylomes of de-etiolating seedling leaves of Zea mays L. (exposed to light for 1, 6, or 12 h) achieved through immunoprecipitation-based high-resolution mass spectrometry (MS). Through the integrated analysis of multiple ubiquitylomes, we identified and quantified 1926 unique ubiquity-lation sites corresponding to 1053 proteins. We analyzed these sites and found five potential ubiqui-tylation motifs, KA, AXK, KXG, AK, and TK. Time-course studies revealed that the ubiquitylation levels of 214 sites corresponding to 173 proteins were highly correlated across two replicate MS exper-iments, and significant alterations in the ubiquitylation levels of 78 sites (fold change >1.5) were detected after de-etiolation for 12 h. The majority of the ubiquitylated sites we identified corre-sponded to substrates involved in protein and DNA metabolism, such as ribosomes and histones. Meanwhile, multiple ubiquitylation sites were detected in proteins whose functions reflect the major physiological changes that occur during plant de-etiolation, such as hormone synthesis/signaling pro-teins, key C4 photosynthetic enzymes, and light signaling proteins. This study on the ubiquitylome of the maize seedling leaf is the first attempt ever to study the ubiquitylome of a C4 plant and provides the proteomic basis for elucidating the role of ubiquitylation during plant de-etiolation.
