Influence of RNA-interfered ribonucleotide reductase M2 on apoptosis and invasion of ovarian cancer cells with drug resistant
10.7619/jcmp.201713018
- VernacularTitle:RNA干扰核糖核苷酸还原酶M2对耐药卵巢癌细胞凋亡及侵袭性的影响
- Author:
Qing LIU
1
;
Ru NIU
;
Songmei JING
Author Information
1. 江苏省徐州市中心医院妇科
- Keywords:
siRNA;
ribonucleotide reductase M2;
ovarian cancer;
drug resistance;
apoptosis
- From:
Journal of Clinical Medicine in Practice
2017;21(13):67-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of RNA-interfered ribonucleotide reductase M2(RRM2)on apoptosis and invasion of ovarian cancer cells with drug resistant.Methods The specific small interfering(siRNA)transfected SKOV3/DDP of RRM2 gene were designed as the interference group.SKOV3/DDP cells,SKOV3/DDP-RRM2 non-specific negative cells were designed as the blank group and negative group.The cell proliferation inhibition rate was detected,RI and drug resistant transfection rate were calculated,the expression of RRM2 mRNA was detected by fluorescence PCR.The effect of siRNA transfection on SKOV3/DDP resistance index and RRM2 protein was analyzed,and the invasion ability of SKOV3/DDP cells was observed with Transwell.Results The transfection rate of the blank group,negative group and interference group was 90％.The drug resistance of DDPto SKOV3/DDP cells was low and gemcitabine was sensitive to SKOV3/DDP cells.DDP and gemcitabine of the DDP on cell half inhibitory concentration(IC50)values showed significant differences among three groups(P<0.05).IC50 values of DDP and gemcitabine on cells in the interference group were significantly lower than the negative group and blank group(P<0.05).The relative expression of SKOV3/DDP cells and SKOV3 cells RRM2 protein showed significantdifferences(P<0.05),and the relative expression was significantly lower in transfection group I,transfection group II and transfection group III than the negative group and blank group(P<0.05).The decrease of relative expression of RRM2 protein was the greatest in the transfection group I(P<0.05).The apoptosis rate was significantly higher in the interference group than the blank group and the negative group,transmembrane cells were fewer than the blank group and the negative group(P<0.05).Conclusion siRNA can effectively inhibit the proliferation and invasion of RRM2 gene in ovarian cancer.It can increase the drug sensitivity of drug resistant cells,especially cells promoting the apoptosis of drug resistant cells induced by DDP.
