Thyroid disruptor p, p'-DDE inhibited the expression of LHX4 and DIS3L protein in Nthy-ori-3-1 cells
10.3760/cma.j.issn.cn121094-20190902-00361
- VernacularTitle:甲状腺干扰物 p， p'-DDE抑制Nthy-ori-3-1细胞LHX4和DIS3L蛋白表达
- Author:
Wei ZOU
1
;
Jiaxin LI
;
Fang XU
;
Hongmei PAN
;
Jianyu ZHOU
;
Hua BAI
;
Qi WANG
Author Information
1. 650500 昆明医科大学公共卫生学院
- Keywords:
p, p'-DDE;
Nthy-ori-3-1;
LHX4;
DIS3L
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(8):561-565
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes of LHX4 and DIS3L mRNA and protein expression in Nthy-ori-3-1 cells after the treatment of thyroid disruptor p, p'-DDE. Methods:Nthy-ori-3-1 cells in logarithmic growth phase were treated with 0, 0.5, 1.0, 2.0 and 5.0 μg/ml p, p'-DDE solution. The growth state and morphology of the cells were observed by microscope. The mRNA levels of LHX4 and DIS3L were detected by real-time fluorescent quantitative PCR, and the protein expression levels of LHX4 and DIS3L were detected by Western blot. Results:when the concentrations of p, p'-DDE were 0, 0.5, 1.0 and 2.0 μg/ml, Nthy-ori-3-1 cells grew normally. There were 33 differential genes in 2.0 μg/ml group, among which 13 genes were down regulated and 20 genes were up-regulated. Compared with the control group, the protein expression levels of LHX4 and DIS3L in 1.0 and 2.0 μg/ml groups were significantly decreased ( P<0.05) , and the relative expression levels of LHX4 and DIS3L protein mRNA in 1.0 μg/ml group were significantly decreased ( P<0.05) . Conclusion:p, p'-DDE can affect the protein expression of LHX4 and dis3l in nthy-ori-3-1 cells.
